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KMID : 0603820090150020153
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Journal of Experimental & Biomedical Science
2009 Volume.15 No. 2 p.153 ~ p.160
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Mechanism of Photodynamic Therapy using 9-hydroxypheophorbide-alpha on HeLa Cell Lines
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Ahn Jin-Chul
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Abstract
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Photodynamic therapy (PDT) is a treatment utilizing the generation of singlet oxygen and other reactive oxygen species (ROS), which selectively accumulate in target cells. The aim of present work is to investigate the photodynamic therapy mechanism of 9-HpbD-a-mediated PDT in HeLa cell lines. We studied the general reactive oxygen species (G-ROS) activation after 9-HpbD-a PDT using fluorescence stain with H₂DCF-DA. G-ROS activation observed after 9-HpbD-a PDT and higher activation condition was 1 hour after PDT at 0.5 ㎍/㎖ 9-HpbD-a concentration. Sodium azide and reduced glutathione (the singlet oxygen quencher) could protect HeLa cells from cell death induced by 9-HpbD-a PDT. But D-mannitol (the hydroxyl radical scavenger) could not protect cell death. Singlet oxygen played a decisive role in 9-HpbD-a PDT induced HeLa cell death. Type Ⅱ reaction was the main type of ROS formation at 9-HpbD-a PDT.
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KEYWORD
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Photodynamic therapy, 9-hydroxypheophorbide-a (9-HpbD-a), Reactive species oxygen (ROS)
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